4UCO

Fragment bound to H.influenza NAD dependent DNA ligase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-31
Detector	RIGAKU CCD
Spacegroup name	P 43 21 2
Unit cell lengths	137.008, 137.008, 55.974
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.330 - 2.500
R-factor	0.222
Rwork	0.220
R-free	0.25350
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	BUSTER-TNT (BUSTER 2.11.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.080	0.340
Number of reflections	14429
<I/σ(I)>	6.7	0.7
Completeness [%]	76.0	63
Redundancy	2	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1