4UCL

X-ray structure and activities of an essential Mononegavirales L- protein domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Collection date	2013-10-06
Spacegroup name	P 21 21 21
Unit cell lengths	78.780, 83.410, 182.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	91.460 - 2.800
R-factor	0.1907
Rwork	0.189
R-free	0.21660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.020
Data reduction software	xia2
Data scaling software	xia2
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	182.920
High resolution limit [Å]	2.800
Rmerge	0.080
Number of reflections	30451
Completeness [%]	99.8
Redundancy	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1