4UCJ

X-ray structure and activities of an essential Mononegavirales L- protein domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-26
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	82.100, 84.530, 181.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.260 - 3.260
R-factor	0.1876
Rwork	0.186
R-free	0.21970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.980
Data reduction software	xia2
Data scaling software	xia2
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	42.260
High resolution limit [Å]	3.260
Rmerge	0.200
Number of reflections	20356
Completeness [%]	99.9
Redundancy	12.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1