4UC2
Crystal structure of translocator protein 18kDa (TSPO) from rhodobacter sphaeroides (A139T mutant) in P212121 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.003 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.083, 95.837, 105.827 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.650 - 2.400 |
| R-factor | 0.2069 |
| Rwork | 0.206 |
| R-free | 0.23630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uc1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.118 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.650 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 29986 | |
| <I/σ(I)> | 10.8 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 8.5 | 293 | 23% PEG 3350, 0.1 M (NH4)2SO4 and 0.1 M Hepes buffer pH 8.5 |
