4UBR

Crystal structure of Pseudomonas aeruginosa N-aetyltransferase PA4534

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-12
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	137.367, 137.367, 200.854
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.700 - 2.210
R-factor	0.19387
Rwork	0.192
R-free	0.22763
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.739
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.210
Number of reflections	96199
<I/σ(I)>	21.9
Completeness [%]	99.6
Redundancy	15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M Calcium acetate, 0.1M Tris HCl pH 7.0, 16% PEG 1000