4UBA

Low-salt structure of protein kinase CK2 catalytic subunit with 4'-carboxy-6,8-bromo-flavonol (FLC26)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-10-20
Detector	PSI PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 43 21 2
Unit cell lengths	126.000, 126.000, 124.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.548 - 2.995
R-factor	0.1897
Rwork	0.187
R-free	0.23860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.552
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	44.550
High resolution limit [Å]	2.995
Number of reflections	20425
<I/σ(I)>	10.6
Completeness [%]	98.5
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	Reservoir: 30 %(w/v) PEG4000, 0.2 M ammonium acetate, 0.1 M trisodium citrate