4UB8
Native structure of photosystem II (dataset-2) by a femtosecond X-ray laser
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL3 |
| Synchrotron site | SACLA |
| Beamline | BL3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-16 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.24 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 123.807, 230.001, 288.502 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.904 - 1.950 |
| R-factor | 0.1894 |
| Rwork | 0.188 |
| R-free | 0.22500 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.439 |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 62.300 |
| High resolution limit [Å] | 1.950 |
| Number of reflections | 586125 |
| <I/σ(I)> | 3.1 |
| Completeness [%] | 98.4 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 290 | PEG, magnesium sulfate |
