4UB4
DNA polymerase beta substrate complex with a templating cytosine and incoming dGTP, 0 s
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-21 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.594, 79.372, 55.465 |
| Unit cell angles | 90.00, 107.50, 90.00 |
Refinement procedure
| Resolution | 25.429 - 1.954 |
| R-factor | 0.1904 |
| Rwork | 0.188 |
| R-free | 0.23490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fms |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.029 |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.990 |
| High resolution limit [Å] | 1.954 | 1.954 |
| Rmerge | 0.068 | 0.513 |
| Number of reflections | 49693 | |
| <I/σ(I)> | 19.6 | 2.2 |
| Completeness [%] | 99.5 | 95.8 |
| Redundancy | 4.8 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 50 mM imidazole, 350 mM sodium chloride, 17% PEG3350, pH 8.0 |
