4UB0
New design for monovalent bispecific IgG through cysteine engineering of the CH1-CL interface
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97931 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.154, 64.045, 82.395 |
| Unit cell angles | 90.00, 129.09, 90.00 |
Refinement procedure
| Resolution | 53.600 - 2.200 |
| R-factor | 0.23308 |
| Rwork | 0.232 |
| R-free | 0.25202 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.027 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.600 | 2.310 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.397 | |
| Number of reflections | 25434 | |
| <I/σ(I)> | 11.6 | 2.8 |
| Completeness [%] | 97.4 | 97.9 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | protein @ 19 mg/ml in 10 mM Tris-HCl, pH 8.0, 100 mM NaCl; 0.018 M of each of the following salt solutions: CaCl2, CoCl2, and CdCl2; 18% PEG 3350 and 5% glycerol |
