4UA8
EUR_01830 (maltotriose-binding protein) complexed with maltotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 99.691, 110.495, 38.515 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.010 - 1.540 |
| R-factor | 0.138 |
| Rwork | 0.137 |
| R-free | 0.16100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Internal model |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.410 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.010 | 1.600 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.081 | 0.175 |
| Number of reflections | 63371 | |
| <I/σ(I)> | 30.19 | 7.16 |
| Completeness [%] | 99.2 | 98.1 |
| Redundancy | 7.4 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 35-40% pentaerythritol propoxylate 5/4 PO/OH, 0.2M NaCl, 0.1M MES pH 5.0 |
