4U9W
Crystal Structure of NatD bound to H4/H2A peptide and CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-09 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.901, 93.288, 100.329 |
| Unit cell angles | 90.00, 96.69, 90.00 |
Refinement procedure
| Resolution | 26.240 - 2.490 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4u9v |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.206 |
| Data reduction software | DENZO |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.102 | 0.552 |
| Number of reflections | 33518 | |
| <I/σ(I)> | 8 | |
| Completeness [%] | 98.8 | 97.6 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 20% PEG 3350, 200 mM sodium malonate |
