4U9L

Structure of a membrane protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-01
Detector	RAYONIX MX225HE
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	62.529, 70.165, 102.644
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.424 - 2.300
R-factor	0.2264
Rwork	0.224
R-free	0.26320
RMSD bond length	0.003
RMSD bond angle	0.734
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Number of reflections	21294
<I/σ(I)>	9.62
Completeness [%]	94.8
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	8	293	PEG600, Tris, Mg-formate, NaCl