4U98
Structure of mycobacterial maltokinase, the missing link in the essential GlgE-pathway (AppCp complex)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 63.000, 73.520, 106.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.648 - 1.150 |
| R-factor | 0.1463 |
| Rwork | 0.145 |
| R-free | 0.16320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4u94 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.026 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.648 | 1.210 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Number of reflections | 173534 | |
| <I/σ(I)> | 21.7 | 1.8 |
| Completeness [%] | 98.6 | 92.6 |
| Redundancy | 4.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | MOPS/sodium HEPES pH 7.5, 0.12 M ethylene glycols (0.03M each of di-ethyleneglycol, tri-ethyleneglycol, tetra-ethyleneglycol, and penta-ethyleneglycol), 30% PEG 500MME/PEG 20K |
