4U94
Structure of mycobacterial maltokinase, the missing link in the essential GlgE-pathway
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 62.947, 73.409, 106.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.741 - 1.473 |
| R-factor | 0.1416 |
| Rwork | 0.140 |
| R-free | 0.17990 |
| Structure solution method | MIR |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.415 |
| Data reduction software | XDS |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.741 | 1.550 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Number of reflections | 84256 | |
| <I/σ(I)> | 24.6 | 7.7 |
| Completeness [%] | 99.9 | 99.2 |
| Redundancy | 10.9 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | MOPS/sodium HEPES pH 7.5, 0.12 M ethylene glycols (0.03M each of di-ethyleneglycol, tri-ethyleneglycol, tetra-ethyleneglycol, and penta-ethyleneglycol), 30% PEG 500MME/PEG 20K |
