4U8G
Crystal structure of 2-keto-3-deoxy-D-gluconate dehydrogenase from Streptococcus agalactiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 83.943, 83.943, 181.101 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.640 - 2.900 |
| R-factor | 0.21477 |
| Rwork | 0.211 |
| R-free | 0.28690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cxr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.219 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.900 |
| Number of reflections | 8964 |
| <I/σ(I)> | 78.5 |
| Completeness [%] | 99.8 |
| Redundancy | 22.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% polyethylene glycol monomethyl ether 550, 0.1 M sodium chloride, and 0.1 M sodium N,N-bis(2-hydroxyethyl)glycine (pH 9.0) |
