4U81
MEK1 Kinase bound to small molecule inhibitor G659
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2007-12-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.987 |
Spacegroup name | P 62 |
Unit cell lengths | 82.083, 82.083, 129.480 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.716 - 2.701 |
R-factor | 0.1694 |
Rwork | 0.167 |
R-free | 0.21830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s9j |
RMSD bond length | 0.008 |
RMSD bond angle | 1.172 |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.790 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.750 | |
Number of reflections | 13584 | |
<I/σ(I)> | 36.2 | 2.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.7 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 291 | The protein was concentrated to 15mg/ml and incubated with 10 fold molar excess inhibitor plus 1mM MgAMP-PNP before crystallization. MEK1 crystals grew from hanging drop vapor diffusion using 12% w/v PEG 8000, 0.4M NH4H2PO4 and 0.1M HEPES pH 6.9 at 18 degC. |