4U7M

LRIG1 extracellular domain: Structure and Function Analysis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.954
Spacegroup name	P 43 21 2
Unit cell lengths	121.040, 121.040, 115.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.957 - 2.757
R-factor	0.207
Rwork	0.205
R-free	0.25330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g1c
RMSD bond length	0.006
RMSD bond angle	0.870
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		2.910
High resolution limit [Å]	2.757	8.140	2.757
Rmerge	0.167	0.045	3.000
Rmeas	0.177	0.048	3.174
Total number of observations	213196
Number of reflections	22552	989	3103
<I/σ(I)>	11.68	36.35	0.88
Completeness [%]	98.3	99.1	89.2
Redundancy	9.4		9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	293	0.8 M sodium succinate pH 7.0