4U7L

LRIG1 extracellular domain: Structure and Function Analysis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.954
Spacegroup name	P 21 21 21
Unit cell lengths	38.940, 93.760, 169.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.378 - 2.300
R-factor	0.1968
Rwork	0.194
R-free	0.24780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xku
RMSD bond length	0.004
RMSD bond angle	0.886
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]			2.390
High resolution limit [Å]	2.300	7.140	2.300
Rmerge	0.132	0.045	2.242
Rmeas	0.143	0.050	2.412
Total number of observations	202942
Number of reflections	28430	1028	3003
<I/σ(I)>	9.21	27.27	0.87
Completeness [%]	99.8	94.8	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	293	16% PEG4000 0.1M Hepes pH7.0 10mM Taurine