4U6U
Crystal Structure of the Cog5-Cog7 complex from Kluyveromyces lactis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075, 0.9790, 0.9792 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 335.838, 335.838, 335.838 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.474 - 3.000 |
| R-factor | 0.1932 |
| Rwork | 0.190 |
| R-free | 0.23090 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.503 |
| Data reduction software | DENZO |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 2.285 | |
| Number of reflections | 33059 | |
| <I/σ(I)> | 26.5 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 28.9 | 29.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | PEG 4000, Tris |
