4U6N
Crystal structure of Escherichia coli DiaA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-04-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 47.490, 73.820, 106.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.650 - 1.910 |
| R-factor | 0.1665 |
| Rwork | 0.165 |
| R-free | 0.19841 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yva |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.887 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.000 | 2.000 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.081 | 0.594 |
| Number of reflections | 14941 | |
| <I/σ(I)> | 13.2 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.9 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.9 | 298 | 0.1 M Tris pH 8.9, 0.25 M MgCl2, and 32-38% PEG3000 |
