4U64

Structure of the periplasmic output domain of the Legionella pneumophila LapD ortholog CdgS9 in the apo state

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-01
Detector	ADSC QUANTUM 210
Wavelength(s)	0.976
Spacegroup name	P 61 2 2
Unit cell lengths	61.581, 61.581, 147.528
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.790 - 2.141
R-factor	0.2217
Rwork	0.219
R-free	0.24300
RMSD bond length	0.003
RMSD bond angle	0.598
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.790
High resolution limit [Å]	2.140
Number of reflections	9611
<I/σ(I)>	35.4
Completeness [%]	98.6
Redundancy	21.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.1 M Bis-Tris (pH=5.0), 14% PEG3350 and 4% v/v 2,2,2-trifluoroethanol