4U62
Trichodysplasia spinulosa-associated polyomavirus (TSPyV) VP1 in complex with 3'-sialyllactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-03 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.240, 152.840, 147.240 |
| Unit cell angles | 90.00, 92.34, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.550 |
| R-factor | 0.15654 |
| Rwork | 0.155 |
| R-free | 0.18028 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4u5z |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.356 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.590 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 412698 | |
| <I/σ(I)> | 13.4 | 2.2 |
| Completeness [%] | 97.8 | 93.5 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM Na-malonate pH 5.0, 10% PEG 3350 |
