4U5X

Structure of plant small GTPase OsRac1 complexed with the non-hydrolyzable GTP analog GMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-14
Detector	RAYONIX MX225HE
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	36.755, 59.087, 64.393
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.540 - 1.900
R-factor	0.15872
Rwork	0.157
R-free	0.20250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2j0v
RMSD bond length	0.019
RMSD bond angle	2.202
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.095	0.284
Number of reflections	11521
<I/σ(I)>	19.7	7.7
Completeness [%]	99.8	100
Redundancy	8.2	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	PEG 6000, MES buffer