4U4U

Crystal structure of Lycorine bound to the yeast 80S ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	435.260, 287.540, 304.020
Unit cell angles	90.00, 98.97, 90.00

Refinement procedure

Resolution	73.990 - 3.000
R-factor	0.2124
Rwork	0.211
R-free	0.25880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3U5B 3U5C 3U5D 3U5E 3U5F 3U5G 3U5H 3U5I
RMSD bond length	0.012
RMSD bond angle	1.637
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: dev_1702))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	73.990		3.100
High resolution limit [Å]	3.000	75.000	3.000
Rmerge	0.295		2.415
Rmeas	0.317		2.593
Total number of observations	11042307
Number of reflections	1468990		137316
<I/σ(I)>	7.02		0.97
Completeness [%]	100.0		100
Redundancy	7.45

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	PEG 20K, KSCN, Mg Acetate, Tris-Acetate, Glycerol, Spermidine