4U4P

Crystal structure of the human condensin SMC hinge domain heterodimer with short coiled coils

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.980
Spacegroup name	P 21 21 21
Unit cell lengths	50.400, 93.980, 102.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.191 - 1.890
R-factor	0.19
Rwork	0.188
R-free	0.23470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3l51
RMSD bond length	0.007
RMSD bond angle	0.974
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.990	46.990	1.930
High resolution limit [Å]	1.890	8.990	1.890
Rmerge	0.066	0.041	0.476
Rpim	0.044	0.024	0.637
Total number of observations	209398	1513	8767
Number of reflections	47639
<I/σ(I)>	10.8	26.9	2.6
Completeness [%]	98.4	91.1	99.5
Redundancy	4.4	3.9	4.3
CC(1/2)	0.997	0.996	0.365

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.1M Tris-HCl pH 8.5, 19% PEG 3350, 50mM NaCl