4U4N
Crystal structure of Edeine bound to the yeast 80S ribosome
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 434.770, 287.660, 303.840 |
| Unit cell angles | 90.00, 98.99, 90.00 |
Refinement procedure
| Resolution | 73.940 - 3.100 |
| R-factor | 0.2045 |
| Rwork | 0.203 |
| R-free | 0.25150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3U5B 3U5C 3U5D 3U5E 3U5F 3U5G 3U5H 3U5I |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.649 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX ((phenix.refine: dev_1702)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 73.950 | 3.200 | |
| High resolution limit [Å] | 3.100 | 75.000 | 3.100 |
| Rmerge | 0.411 | 1.788 | |
| Rmeas | 0.432 | 1.997 | |
| Total number of observations | 12947660 | ||
| Number of reflections | 1329717 | 120500 | |
| <I/σ(I)> | 6.52 | 0.88 | |
| Completeness [%] | 100.0 | 100 | |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | PEG 20K, KSCN, Mg Acetate, Tris-Acetate, Glycerol, Spermidine |
