4U47
Octameric RNA duplex soaked in terbium(III)chloride
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-27 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.64 |
Spacegroup name | H 3 |
Unit cell lengths | 46.506, 46.506, 56.627 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.820 - 1.952 |
R-factor | 0.1755 |
Rwork | 0.172 |
R-free | 0.20670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4u3l |
RMSD bond length | 0.004 |
RMSD bond angle | 0.783 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.820 | 2.020 |
High resolution limit [Å] | 1.952 | 1.952 |
Rmerge | 0.050 | 0.436 |
Number of reflections | 3225 | |
<I/σ(I)> | 33.19 | 5.35 |
Completeness [%] | 96.8 | 89.57 |
Redundancy | 9.8 | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 28% PEG 400, 0.2 M calcium chloride, 0.002 M spermine, 0.05 M hepes sodium buffer pH 7.5 |