4U3P
Octameric RNA duplex co-crystallized with strontium(II)chloride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-31 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.60 |
| Spacegroup name | H 3 |
| Unit cell lengths | 45.480, 45.480, 57.980 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.580 - 1.866 |
| R-factor | 0.1852 |
| Rwork | 0.180 |
| R-free | 0.22910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4u3l |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.776 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.580 | 1.932 |
| High resolution limit [Å] | 1.866 | 1.866 |
| Rmerge | 0.036 | 0.471 |
| Number of reflections | 3728 | |
| <I/σ(I)> | 34.84 | 4.06 |
| Completeness [%] | 99.8 | 97.96 |
| Redundancy | 9.4 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 28% PEG 8000, 0.2 M strontium chloride, 0.2 M ammonium acetate, 0.01 M magnesium acetate, 0.05 M sodium cacodylate buffer pH 6.5 |
