4U3L
octameric RNA duplex co-crystallized in calcium(II)chloride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-27 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.61 |
| Spacegroup name | H 3 |
| Unit cell lengths | 47.671, 47.671, 51.885 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.305 - 1.482 |
| R-factor | 0.1708 |
| Rwork | 0.170 |
| R-free | 0.18840 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.956 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.300 | 1.535 |
| High resolution limit [Å] | 1.482 | 1.482 |
| Rmerge | 0.036 | 0.410 |
| Number of reflections | 14194 | |
| <I/σ(I)> | 25.82 | 3.47 |
| Completeness [%] | 99.8 | 99.45 |
| Redundancy | 5.3 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 28% PEG 400, 0.2 M calcium chloride, 0.002 M spermine, 0.05 hepes sodium buffer pH 7.5 |
