4U37
Native 7mer-RNA duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2012-04-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 37.478, 38.490, 30.408 |
| Unit cell angles | 90.00, 110.46, 90.00 |
Refinement procedure
| Resolution | 28.490 - 1.550 |
| R-factor | 0.20385 |
| Rwork | 0.203 |
| R-free | 0.22796 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 434d |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.997 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.124 | 0.173 |
| Number of reflections | 5375 | |
| <I/σ(I)> | 12.3 | 6.35 |
| Completeness [%] | 95.1 | 73.7 |
| Redundancy | 5.3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 10% MPD, 40 mM Na cacodylate pH 6.0, 12 mM spermine tetra-HCl and 80 mM NaCl |
