4U31

Sco GlgEI-V279S in Complex with maltose-C-phosphonate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.08
Spacegroup name	P 41 21 2
Unit cell lengths	113.556, 113.556, 315.142
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.685 - 1.849
R-factor	0.1646
Rwork	0.164
R-free	0.19430
RMSD bond length	0.011
RMSD bond angle	1.349
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.800
Number of reflections	175910
<I/σ(I)>	37.6
Completeness [%]	100.0
Redundancy	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.2 M sodium citrate pH 7.0 and 10 % PEG 3350