4U2Z

X-ray crystal structure of an Sco GlgEI-V279S/1,2,2-trifluromaltose complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.08
Spacegroup name	P 41 21 2
Unit cell lengths	113.297, 113.297, 314.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.584 - 2.260
R-factor	0.1647
Rwork	0.163
R-free	0.19930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.094
Phasing software	HKL-3000
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.260
Number of reflections	96584
<I/σ(I)>	26
Completeness [%]	99.9
Redundancy	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.2 M sodium citrate pH 7.0 and 10 % PEG 3350