4U2Y
Sco GlgEI-V279S in Complex with Reaction Intermediate Azasugar
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.08 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 113.829, 113.829, 314.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.558 - 2.483 |
R-factor | 0.1769 |
Rwork | 0.175 |
R-free | 0.21650 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.352 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.483 |
Number of reflections | 69481 |
<I/σ(I)> | 31.3 |
Completeness [%] | 94.0 |
Redundancy | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2 M sodium citrate pH 7.0, and 10 % PEG 3350 |