4U2I

N-terminal domain of C. Rheinhardtii SAS-6 homolog bld12p Q93E G94D K146R Q147R (NN23)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-07-04
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	40.170, 96.630, 142.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.315 - 2.281
R-factor	0.2079
Rwork	0.206
R-free	0.24920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3q0y
RMSD bond length	0.003
RMSD bond angle	0.682
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.280	2.280
Rmerge		0.590
Number of reflections	25555
<I/σ(I)>	21.46	3.47
Completeness [%]	97.8	73.2
Redundancy	9.8	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	293	PEG 3350, MES mononhydrate