4U10
Probing the structure and mechanism of de-N-acetylase from aggregatibacter actinomycetemcomitans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-12-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.240, 67.100, 101.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.480 - 2.050 |
| R-factor | 0.1654 |
| Rwork | 0.163 |
| R-free | 0.21956 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vus |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.746 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.100 | |
| Number of reflections | 28980 | |
| <I/σ(I)> | 34.1 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.4 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% PEG 6000, 0.01M ZnCl2, 0.1M HEPES |
