4U0V
Crystal structure of YvoA from Bacillus subtilis in complex with glucosamine-6-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-05 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.91841 |
Spacegroup name | H 3 2 |
Unit cell lengths | 98.499, 98.499, 355.133 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.647 - 2.051 |
R-factor | 0.2021 |
Rwork | 0.200 |
R-free | 0.24780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wv0 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.137 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.650 | 2.120 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.058 | 0.655 |
Number of reflections | 42017 | |
<I/σ(I)> | 19.39 | 2.43 |
Completeness [%] | 99.7 | 98.6 |
Redundancy | 4.3 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292.15 | 100 mM Na-HEPES salt, 200 mM ammonium chloride, 25 % (v/v) glycerol ethoxylate |