4U0U
Wild type eukaryotic fic domain containing protein with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-06 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 77.750, 109.040, 131.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.620 - 2.980 |
| R-factor | 0.2019 |
| Rwork | 0.199 |
| R-free | 0.25610 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.620 | 3.250 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Rmerge | 0.410 | |
| Number of reflections | 20620 | |
| <I/σ(I)> | 11.8 | 5.3 |
| Completeness [%] | 84.0 | 43.5 |
| Redundancy | 14 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 20% PEG 3350, 200mM Na K tartrate, 100mM Bis-Tris-Propane 7.5 |
