4U07
ATP bound to eukaryotic FIC domain containing protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-26 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.984 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.254, 76.114, 92.189 |
| Unit cell angles | 90.00, 107.28, 90.00 |
Refinement procedure
| Resolution | 48.880 - 2.640 |
| R-factor | 0.2104 |
| Rwork | 0.208 |
| R-free | 0.25810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cuc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.880 |
| High resolution limit [Å] | 2.640 |
| Number of reflections | 27626 |
| <I/σ(I)> | 7.1 |
| Completeness [%] | 98.1 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 20% peg 3350, 200mM Na K tartrate, 100mM Bist-Tris Propane 7.5 |
