4U05
Homodimeric Single Domain Antibody (sdAb) against Staphylococcal enterotoxin B (SEB) S74A Variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2012-04-08 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 67.193, 72.169, 117.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.580 - 2.500 |
| R-factor | 0.21045 |
| Rwork | 0.209 |
| R-free | 0.23876 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tyu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.124 |
| Data scaling software | PROTEUM PLUS |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.580 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.190 | 0.437 |
| Number of reflections | 10160 | |
| <I/σ(I)> | 8.05 | 3.95 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 7.61 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 2.5 | 290 | 0.1 M Citric Acid pH 2.5 1.6 M Ammonium Sulfate 20% Glycerol |
