4U04
Structure of a eukaryotic fic domain containing protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-29 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.186, 76.809, 93.195 |
| Unit cell angles | 90.00, 108.05, 90.00 |
Refinement procedure
| Resolution | 43.810 - 2.480 |
| R-factor | 0.2193 |
| Rwork | 0.218 |
| R-free | 0.24480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cuc |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.193 | 2.610 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.088 | 0.854 |
| Number of reflections | 33248 | |
| <I/σ(I)> | 6.1 | |
| Completeness [%] | 97.7 | 97.7 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 20% PEG 3350, 200mM Na K Tartrate, 100mM Bis-Tris Propane 7.5 |
