4TZY

Vibrio vulnificus Adenine Riboswitch Variant, grown in both Sr2+ and Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-01-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 21 21 2
Unit cell lengths	49.283, 152.313, 24.995
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.375 - 2.570
R-factor	0.2081
Rwork	0.203
R-free	0.25080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1y26
RMSD bond length	0.001
RMSD bond angle	0.324
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.400	2.660
High resolution limit [Å]	2.570	2.570
Rmerge	0.077	0.641
Number of reflections	6534
<I/σ(I)>	15.3	2
Completeness [%]	99.8	99.7
Redundancy	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	Mg, PEG, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K