4TZX

Vibrio Vulnificus Adenine Riboswitch variant, grown in Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-01-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 21 21 2
Unit cell lengths	49.641, 152.839, 24.991
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.210 - 2.010
R-factor	0.2272
Rwork	0.224
R-free	0.25660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1y26
RMSD bond length	0.001
RMSD bond angle	0.349
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.210	2.130
High resolution limit [Å]	2.010	2.010
Rmerge	0.065	0.993
Number of reflections	13466
<I/σ(I)>	21.1	2.1
Completeness [%]	99.6	99.3
Redundancy	5.7	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	Mg, PEG, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K