4TZS

Structure of C. elegans HTP-2 bound to HIM-3 closure motif, P212121 form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	.979
Spacegroup name	P 21 21 21
Unit cell lengths	66.236, 82.676, 118.703
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.039 - 2.550
R-factor	0.2303
Rwork	0.229
R-free	0.26420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.771
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.550
Number of reflections	21690
<I/σ(I)>	15.8
Completeness [%]	99.0
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298.15	15-22% PEG 3350