4TZP

As Grown, Untreated Co-crystals of the Ternary Complex Containing a T-box Stem I RNA, its cognate tRNAGly, and B. subtilis YbxF protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	C 2 2 21
Unit cell lengths	108.730, 108.749, 291.811
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.630 - 8.503
R-factor	0.2779
Rwork	0.267
R-free	0.37330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lck
RMSD bond length	0.004
RMSD bond angle	0.818
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.630
High resolution limit [Å]	8.500	8.500
Rmerge	0.083	1.080
Number of reflections	1651
<I/σ(I)>	10.6	1.7
Completeness [%]	99.9	100
Redundancy	6.7	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	50 mM Bis-Tris (HCl) pH 6.5, 300 mM Li2SO4, and 20% (w/v) PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K