4TZM

C. elegans HTP-2 bound to HTP-3 closure motif 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	47.676, 66.005, 94.649
Unit cell angles	90.00, 90.27, 90.00

Refinement procedure

Resolution	28.465 - 2.300
R-factor	0.2036
Rwork	0.203
R-free	0.21640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tzl
RMSD bond length	0.003
RMSD bond angle	0.647
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.300
Number of reflections	24989
<I/σ(I)>	14.5
Completeness [%]	93.8
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	15-22% PEG 3350