4TZG

Crystal structure of eCGP123, an extremely thermostable green fluorescent protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-05-15
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.542
Spacegroup name	P 1
Unit cell lengths	74.630, 75.380, 84.513
Unit cell angles	90.96, 89.82, 104.03

Refinement procedure

Resolution	29.850 - 2.100
R-factor	0.16923286509
Rwork	0.167
R-free	0.20330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3adf
RMSD bond length	0.004
RMSD bond angle	0.929
Data reduction software	MOSFLM (1.9_1685)
Data scaling software	SCALA
Refinement software	PHENIX (1.9_1685)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.850
High resolution limit [Å]	2.100
Number of reflections	98476
<I/σ(I)>	9.6
Completeness [%]	94.4
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Tris-HCl pH 8.0, 22.5% (w/v) PEG 3350, 0.2 M MgCl2