4TZD

Crystal structure of Canavalia maritima lectin (ConM) complexed with interleukin - 1 beta primer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-15
Detector	MAR CCD 165 mm
Wavelength(s)	1.459
Spacegroup name	F 2 3
Unit cell lengths	192.230, 192.230, 192.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.240 - 3.200
R-factor	0.25062
Rwork	0.247
R-free	0.31494
RMSD bond length	0.016
RMSD bond angle	1.657
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.060
High resolution limit [Å]	3.200
Number of reflections	9248
<I/σ(I)>	5.7
Completeness [%]	94.7
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M Magnesium chloride hexahydrate, 0.1 M HEPES sodium 7.5, 30% v/v 2-Propanol