4TZ6

DEAD-box helicase Mss116 bound to ssRNA and UDP-BeF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	89.757, 126.506, 55.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.211 - 3.209
R-factor	0.2221
Rwork	0.220
R-free	0.26980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3i5x
RMSD bond length	0.003
RMSD bond angle	0.753
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1583))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.211	3.360
High resolution limit [Å]	3.209	3.209
Rmerge	0.198	0.665
Number of reflections	10514
<I/σ(I)>	5.6	2.5
Completeness [%]	96.7	95
Redundancy	8.2	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	0.1 M DL-malic acid, pH 7.0, 12 % PEG 3350