4TYW

DEAD-box helicase Mss116 bound to ssRNA and ADP-BeF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	89.829, 126.260, 55.545
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.243 - 2.197
R-factor	0.218
Rwork	0.216
R-free	0.25380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3i5x
RMSD bond length	0.003
RMSD bond angle	0.696
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.243	2.240
High resolution limit [Å]	2.197	2.200
Rmerge	0.097	0.603
Number of reflections	32642
<I/σ(I)>	6.8	1.54
Completeness [%]	99.4	97.7
Redundancy	6.8	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M HEPES, pH 7.5, 2 % tacsimate pH 7.0, 20 % PEG 3350