4TYN

DEAD-box helicase Mss116 bound to ssDNA and ADP-BeF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	77
Detector technology	CCD
Collection date	2012-03-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	90.389, 126.189, 55.231
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.150 - 2.959
R-factor	0.1986
Rwork	0.196
R-free	0.24440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3i5x
RMSD bond length	0.003
RMSD bond angle	0.751
Refinement software	PHENIX ((phenix.refine: dev_1583))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.150	3.050
High resolution limit [Å]	2.959	3.000
Rmerge	0.198	0.612
Number of reflections	13129
<I/σ(I)>	8.55	1.46
Completeness [%]	94.6	88
Redundancy	5.5	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	0.2 M ammonium acetate, 20 % PEG 3350